Table 1.
List of primary antibodies used for immunohistochemical staining of skeletal muscle biopsy tissue and cultured muscle cells
| Specificity | Host species and clone | Incubation and dilution or concentration | Supplier |
|---|---|---|---|
| αB-crystallin | Rabbit polyclonal | 1 h; 1 : 1000 dilution | Serotec, Oxford, UK |
| APP | Goat polyclonal | 1 h; 10 μg/ml | R&D, Minneapolis, MN |
| β-amyloid | Mouse clone 6E10 | 24 h/4°C; 10 μg/ml | Signet, Dedham, MA, USA |
| CCL-3 | Goat polyclonal | 24 h/4°C; 20 μg/ml | R&D |
| CCL-4 | Goat polyclonal | 24 h/4°C; 5 μg/ml | Abcam, Cambridge, MA |
| CXCL-9 | Goat polyclonal | 24 h/4°C; 10 μg/ml | R&D |
| Desmin | Rabbit polyclonal | 1 h; 30 μg/ml | Abcam |
| IFN-γ | Mouse clone 25723 | 24 h/4°C; 5.6 μg/ml | R&D |
| IL-1β | Rabbit polyclonal | 24 h/4°C; 10 µg/ml | Abcam |
| MHC-I | Rat clone YTH 862.2 | 1 h; 5 μg/ml | Serotec |
| NCAM | Mouse clone Eric-1 | 1 h; 2 μg/ml | Labvision/Neomarkers, Fremont, CA |
| TGF-β | Mouse clone MAB1032 | 1 h; 0.5 μg/ml | Chemicon, Temecula, CA |
| Ubiquitin | Rabbit polyclonal | 30 min; 15 μg/ml | DAKO, Carpinteria, CA |