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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 4;58(2):184–193. doi: 10.1016/j.pep.2007.11.010

Figure 3. Affinity and number of binding sites of his-ACBP with cis-parinaroyl-CoA (cPNCoA) and cis-parinaric acid (cPNA).

Figure 3

Scatter plot and curve fitting of maximal fluorescence intensity (ex 310 nm, em 410 nm) with increasing of cPNCoA (A) and cPNA (A inset) in presence of 30nM his-ACBP. Reverse titrations of constant amounts (50nM) of cPNCoA (B) and cPNA (B inset) with increasing concentrations of his-ACBP indicated saturation at a molar ratio of 1 with cPNCoA, and nonspecific binding for cPNA. C. UV spectra of cPNCoA (4 μM) in potassium phosphate buffer pH 7.4 (solid), cPNCoA spectra with increasing his-ACBP (0.4-16 μM) are represented by dashed and dotted lines. D. Ratios of absorbance of valley 1 (V1)/peak 2 (P2) from spectra in C plotted vs protein/ligand molar ratio. E. Wavelengths of peak 2 (P2) in C plotted vs protein/ligand molar ratio.