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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 4;58(2):184–193. doi: 10.1016/j.pep.2007.11.010

Figure 4. Structural and functional characterization of Cy5-labeled his-ACBP.

Figure 4

A. SDS-PAGE of Cy5-his-ACBP and his-ACBP. B. Western blotting of Cy5-his-ACBP and his-ACBP, 500ng of protein per lane. C. Far UV CD spectrum of his-ACBP and Cy5-his-ACBP. All CD studies were performed with 4μM protein. D. Titration and binding curve of Cy5-his-ACBP with cis-parinaroyl-CoA.