. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: Protein Expr Purif. 2007 Dec 4;58(2):184–193. doi: 10.1016/j.pep.2007.11.010

Table 2. Effect of his-tagging on ACBP enhancement of microsomal glycerol-3-phosphate acyltransferase (GPAT) and acyl CoA synthetase (ACS).

ACBP	μM	GPAT (pmol/min/mg microsomal protein)	ACS (% fatty acid converted to fatty acyl CoA)
None		176 ± 22	21.6 ± 0.4
ACBP	10	564 ± 118	ND
ACBP	20	574 ± 79	30.8 ± 0.1
His-ACBP	10	620 ± 128	ND
His-ACBP	20	628 ± 56	30.0 ± 0.6

ND, not determined. Values represent the Mean ± SE, n=3 or 4.