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LARP7 co-sediments with the 7SK snRNP before and after P-TEFb and HEXIM release. (A) Glycerol gradient sedimentation analysis of untreated HeLa cells. Western blotting was performed with the indicated antibodies. (B) Gradient analysis of cells treated with 500 nM Flavopiridol for 1 h. (C) Northern blot of 7SK from gradient samples used in A and B. (D) Ethidium bromide stain of the RNA gels in C prior to northern transfer.
