Figure 2.

Non-specific DNA cleavage and non-cleavage by singly expressed designed meganuclease monomer variants under different salt conditions. Approximately 3.75 µM of each purified protein was incubated with 3 nM of purified plasmid (pre-linearized with XmnI), containing either the QAN homodimer site (GTT/AAC), the KTG homodimer DNA site (CCT/AGG) or a hybrid site QAN/KTG site (Q-K: GTT/AGG). The concentration of NaCl was either (A) 50 mM or (B) 225 mM. Arrows indicate the uncut target DNA (3.2 kb) or the two bands resulting from digestion (1.1 and 2.1 kb). An asterisk (*) marks control lanes with DNA alone. One-kilobase ladders (Fermentas) are marked by M.