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. 2008 Feb 16;36(7):2208–2218. doi: 10.1093/nar/gkn060

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The Ni-NTA-agarose pull-down assay. (A) A schematic diagram of the assay. (B) Pull-down assay using His₆-tagged histones. hNap1 (lanes 3–5) or hNap2 (lanes 7–9) was mixed with His₆-tagged H3.1/H4 (lanes 4 and 8) or His₆-tagged H3t/H4 (lanes 5 and 9), and the proteins bound to the Ni-NTA agarose beads were analyzed by 15% SDS–PAGE with CBB staining. Lanes 2 and 6: the inputs (25%) of hNap1 and hNap2, respectively. (C) Pull-down assay using His₆-tagged hNap1 and hNap2. H3.1/H4 (lanes 3–5) or H3t/H4 (lanes 7–9) was mixed with His₆-tagged hNap1 (lanes 4 and 8) or His₆-tagged hNap2 (lanes 5 and 9), and the proteins bound to the Ni-NTA agarose beads were analyzed by 15% SDS–PAGE with CBB staining. Lanes 2 and 6: the inputs (25%) of H3.1/H4 and H3t/H4, respectively.