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SDS/PAGE of the R. rhodochrous J1 wild-type and mutant amidases in the supernatants of extracts of each E. coli transformant. The supernatants of extracts prepared from each transformant by the procedure described in Materials and Methods were loaded onto SDS/PAGE gels. The amidase bands are indicated by an arrow. Lanes: M, molecular mass standards; 1, the wild-type amidase; 2, the D191N amidase; 3, the D191E amidase; 4, the S195A amidase; and 5, the C203A amidase.
