Table 2.
Induction of luciferase by noncytopathic replicons or SIN virus
Induction | RLU/106 cells* |
---|---|
None | 1.9 ± 0.3 × 104 |
SINrep18, 5 days after transfection | 5.1 ± 1.7 × 107 |
SINrep18, 9 days after transfection | 7.0 ± 0.9 × 107 |
SIN virus, moi 0.1 | 3.3 ± 1.0 × 106 |
SIN virus, moi 1.0 | 1.7 ± 0.5 × 107 |
Luciferase activity was determined in lysates of puromycin-selected cell populations (for noncytopathic replicons) or 8 h after infection at the indicated moi (with SIN virus, as determined by using BHK monolayers). The uninduced control reflects basal luciferase expression. Values shown reflect the mean and SD for three replicate experiments. RLU, relative light units.