Table 2.

Summary of P50 and n valuesa

	No additions		+NaCl (200 mM)		+DPG (4 mM)
Hemoglobinb	P50	n	P50	n	P50	n
HbA	12	2.5	22	2.9	28	2.9
HbFc	7	2.7			12	2.8
L3F(β)	10	2.3	18	1.9	31	1.6
V11I(β)	9	2.5	18	2.6	25	2.6
L3F(β)/V11I(β)	11	2.9	22	2.4	40	2.0
P5E(β)	12	2.7	19	2.7	27	2.5
V1G(β)/P5E(β)	12	2.7	21	2.3	27	2.1
V1G(β)/P5A(β)	11	2.8	21	2.6	31	2.5
V1G(β)/P5E(β)/E7D(β)	11	2.8	23	2.8	34	2.5
V1G(β)/P5E(β)/E7D(β)/D21E(β)/E22D(β)	11	2.9	26	2.7	38	2.6

^a Average values of three to five determinations.

^b The final Hb concentration was 0.8 mM as tetramer for all experiments.

^c From Chen et al. 2000.