Fig. 1.

Substrate capacity of GAPDH in tTG-mediated cross-links. SDS-PAGE gel Coomassie blue stained (A), fluorescence (B) and corresponding Western Blot analysis performed using anti-tTG antibodies (C) of the in vitro incubation of rabbit muscle GAPDH (50 μg) with dansylated SubP (18 μg) in the presence of purified tTG (5 μg) at 37°C for 1, 4, and 18 h (lanes 4, 5, and 6, respectively). GAPDH and purified tTG were used as controls (lanes 1 and 2, respectively). As additional control, GAPDH was incubated at 37°C for 18 h in the presence of tTG (lane 3). Protein marker levels are indicated.