Figure 2.

Recognition of cells treated with gp120 anthrax toxin fusions by gp120-specific cytotoxic lymphocytes. The mouse mastocytoma cell line P815 was incubated with wild-type protective antigen (WPA) or mutant PACFD (MPA) and/or LF254-gp120 fusion proteins (50 ng/ml). P815 cells pulsed with 1.0 μM P18IIIB peptide served as a control. After 12 hr, the cells were washed and the treated P815 cells were labeled with ⁵¹Cr. Labeled P815 cells were then mixed at different ratios with the CTL cell line 9.23.3, which recognizes the peptide sequence RGPGRAFVTI from gp120. Killing of the target cell population was determined by measuring release of ⁵¹Cr into the medium (% lysis). Background lysis was determined with untreated, ⁵¹Cr labeled, P815 cells. These data are the replicate of three experiments.