Figure 3.

Inhibition of fusion protein processing by lactacystin. P815 cells were treated with 1 ng/ml of the GST-LF254-gp120 fusion protein alone and with PA (WPA) or mutant PA–D (DELPA) in the presence (B) or absence (A) of 10 μM lactacystin. ⁵¹Cr-labeled target cells were mixed with α15–12 CTLs, and specific lysis was measured. Target cells were treated with the P18IIIB peptide as a positive control for cell lysis. These data are the representative of three experiments.