Figure 1.

Expression of BARD1 polypeptides during cell cycle progression. Contact-inhibited T24 cells were released from growth arrest by replating at low density. Synchronized cell populations were then lysed at the indicated time points, and equivalent aliquots of each lysate were subjected to Western analyses with antibodies specific for CDK2, cyclin A, BRCA1, and BARD1. The cell cycle distribution of the synchronized cultures at each time point was estimated by FACS analysis, as tabulated beneath the immunoblots.