Figure 3.

Cell cycle specificity of BARD1 immunostaining. Synchronized populations of T24 cells were costained with propidium iodide and either the BRCA1-specific MS110 monoclonal antibody (A–D) or the BARD1-specific monoclonal antibody (E–H). The composite images of two-color staining are illustrated. The cells were harvested at either 8 hr (A and E) or 20 hr (B–D and F–H) after synchronous release from growth arrest. In some experiments the monoclonal antibodies were preabsorbed with an excess of the BRCA1 immunogen (GST-BR304; C), the BARD1 immunogen (GST-EE; G) or the parental GST polypeptide (D and H).