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BARD1 is a component of the BRCA1 nuclear dots. Asynchronous HBL-100 cells were costained with an affinity-purified BARD1-specific rabbit antiserum (red, A and D) and either a BRCA1-specific (green, B) or PML-specific (green, E) monoclonal antibody. The composite images of two-color staining demonstrate that BARD1 colocalizes with BRCA1 (C) but not with PML (F).
