Figure 5.

BARD1 polypeptides reside within the nuclear compartment of G₁- and S-phase cells. Whole cell (W), nuclear (N), cytoplasmic (C), and membrane (M) fractions were prepared from unsynchronized T24 cells (A–D), as well as from contact-inhibited cells that had been released from growth arrest for either 8 hr or 20 hr (E–H). Equivalent aliquots of each fraction were then subjected to Western analyses with antibodies specific for BRCA1 (A and E), BARD1 (B and F), nuclear matrix protein NuMA (C and G), and α-tubulin (D and H).