Figure 4.
Nascent blood vessels of intracerebral tumors or hemorrhages formed by parental U87MG cells (a and b), VEGF121-overexpressing cells (c and d), VEGF165-overexpressing cells (e and f), and VEGF189-overexpressing cells (g and h). Immunohistochemistry was performed with a monoclonal anti-CD31 antibody (MEC13; PharMingen) on cryostat mouse brain slices. Original magnifications: a, c, e and g: ×10; b, d, f and H: ×200. The photographs were captured into computer files and contrast-enhanced using Adobe Photoshop. The experiments were carried out as described in the legend of Fig. 3.