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Overexpression of VEGF189 in U87MG cells enhances angiogenicity and tumorigenicity in mouse brain. (a and b) Immunohistochemical staining with an anti-CD31 mAb. Intracerebral tumors were formed by the parental U87MG cells (a) or the VEGF189-overexpressing cells (b). Original magnification, ×200. The experiments were repeated five times with similar results. Four to six nude mice in each group were implanted intracerebrally and the times for sacrificing the mice are indicated.
