Table 4.

PCR and hybridization test of DNA diversity among H. pylori strains

Clone	Size, bp	J166	26695	11637	11638	N6	60190	J170	J238	1480	84-183	HP1	WV99	MO19	2002	TX30A
C2	260	++	−−	++	−−	−−	−−	−−	−+	−+*	−−	−+	−−	++	−+	−+
F3	340	++	−−	−−	−−	−−	−−	−−	−−	++	++	−−	−−	−−	++	−−
A3	590	++	−−	++	+−	++	+−	++	++	+−	+−	−−	−−	−−	++	−−
H5	565	++	−−	−+	−+	−+	−−	−+	−−	−+	++	−+	−+	−−	−−	−−
F1	220	++	−−	−+	++	−−	−−	−+	−−	−+	++	−+*	−−	−+	−−	−−
G7	300	++	−−	−−	−+	−−	−−	−−	−−	−−	−−	−−	−−	−−	−−	+−
D3	600	++	−+*	++	−+	++	−+*	++	−+	−+	−+	−+	−+	−+	−+	−+
A7	410	++	−−	++	−−	−+*	−−	−+	−+	−−	−−	−+	−−	++	−−	++
B2	300	++	−−	−−	−−	−−	−−	++	−−	++	−−	−+	−−	++	++	++
C8	300	++	−−	++	++	++	−−	−−	−+	−−	++	−+	++	−+	++	++
G4	410	++	−−	−−	−−	−−	−−	−+	−−	++	++	−−	++	−−	−−	−−

11637 and 11638 designate NCTC11637 and NCTC11638 (NCTC, National Collection of Type Cultures). In each column, the first entry is the result of PCR of the indicated H. pylori strain with the clone-specific primers listed in Table 2. The second entry is the result of Southern blot hybridization with the clone. The designation +* indicates cases of positive hybridization at low stringency only, whereas + indicates positive hybridization at both low and high stringency. In addition, PCR tests with primers specific for the F7 clone yielded products with J166 (positive control), NCTC11637, N6, WV99, 2002, and TX30A, but not 26695 (negative control) nor any of the other eight strains tested.