Table 1.
Plasmids used in this study
| Plasmid | Description | Ref. |
|---|---|---|
| pUC119 | pUC, Apr | 15 |
| pBC SK+ | ColEl, Cmr | Stratagene |
| pBC KS+ | ColEl, Cmr | Stratagene |
| pCR-Script | ColEl, Apr | Stratagene |
| pUC4K | pUC, AprKmr | Pharmacia |
| R702 | Helper plasmid for triparental conjugation, KmrSmrTcr | 16 |
| pCP11 | E. coli–F. johnsoniae shuttle plasmid, pUC ori, AprEmr* | 10 |
| pCP17 | E. coli–F. johnsoniae shuttle cosmid, pUC ori, AprTcrEmr* | 10 |
| pLYL03 | Bacteroides–Flavobacterium suicide vector used to make chromosomal insertions, pUC ori, AprEmr* | 17 |
| pLYL001 | Bacteroides–Flavobacterium suicide vector, pUC ori, AprTcr* | 18 |
| pCP23 | E. coli–F. johnsoniae shuttle plasmid, AprTcr* | This study |
| pCP100 | Cosmid clone complementing F. johnsoniae UW 102-9, AprEmr* | This study |
| pSAR2 | 4.5-kbp XbaI/ScaI fragment of pCP100 containing gldA cloned into the SmaI and XbaI sites of pUC119, Apr | This study |
| pCP25 | 4.5-kbp XbaI/SacI fragment of pSAR2 containing gldA cloned into the XbaI and SacI sites of pCP11, AprEmr* | This study |
| pCP105 | 2-kbp XbaI/BamHI fragment of pCP100 cloned in pCP11, AprEmr* | This study |
| pCP104 | 5-kbp BamHI/SalI fragment of pCP100 cloned in pCP11, AprEmr* | This study |
| pMM114 | HindIII/BamHI fragment of gldA cloned in pLYL03, AprEmr* | This study |
| pSA10 | 1.3-kbp PCR product containing gldA cloned into the SrfI site of pCR-Script, Apr | This study |
| pSA11 | 1.3-kbp SacI/XbaI fragment of pSA10 containing gldA cloned in pCP11, AprEmr* | This study |
| pSA20 | 1.3-kbp SacI/XbaI fragment of pSA10 containing gldA cloned into pBC SK+, Cmr | This study |
| pSA21 | 1.3-kbp XbaI/KpnI fragment of pSA20 containing gldA cloned into pCP23, AprTcr* | This study |
| pDH107 | 605-bp ClaI/HindIII fragment of orf1 cloned into the ClaI and HindIII sites of pUC4K, Apr | This study |
| pDH109-5 | 1.5-kbp PstI fragment of pDH107 which contains the entire 605 bp ClaI/HindIII fragment of orf1 and 740 bp of flanking DNA from the kanamycin resistance gene cloned into pLYL03, Apr Emr* | This study |
Unless indicated otherwise, antibiotic resistances are those expressed in E. coli.
*
Antibiotic resistance is expressed in F. johnsoniae but not in E. coli.