Table 4.
Determination of IFN-γ, IL-2, IL-6, and TNF-α with SAC, SEA, or PMA plus ionomycin using the patient’s cells
| Stimulant | IFN-γ
|
IL-2
|
IL-6
|
TNF-α
|
IL-12 p70*
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| KC | C | KC | C | KC | C | KC | C | KC | C | |
| SAC | ≤3 | 1,104 | 37 | ≤7 | 156 | 10,000 | 1,138 | 13,568 | ≤5 | 32 |
| SEA | 241 | 1,146 | 1,208 | 2,472 | 156 | 600 | 11,295 | 10,633 | ≤5 | 441 |
| PMA + iono | 1,170 | 1,138 | 2,619 | 2,381 | 10,000 | 10,000 | 9,971 | 6,748 | ≤5 | ≤5 |
| Medium | ≤3 | ≤3 | ≤7 | ≤7 | ≤3 | ≤3 | ≤3 | 27 | ≤5 | ≤5 |
PBMCs from the patient (KC) or control (C) were incubated with SAC, SEA, or PMA plus ionomycin (iono) for 24 h. Cytokine yields (pg/ml) in the culture supernatants were measured by ELISA. Results are expressed as the mean of triplicate cultures. The deviation from the mean is always <10% of the mean value.
*
IL-12 determinations were performed in three separate experiments. A 48-h culture of IL-12 supernatant also was tested. The result of KC’s IL-12 at 48 h was ≤5. C IL-12 was 241 pg/ml.