FIG. 3.

Effects of estrogen on changes in pHo. The hECE cells generated from tissues of premenopausal (Pre-M) and postmenopausal (Post-M) women were plated on filters and shifted to steroid-free medium for 1 day, and then maintained in the same medium for 2 additional days in the absence (SFM) or presence of 10 nM 17β-estradiol (added to both the luminal and contraluminal solutions) (+E₂). For assays, cultures were shifted to basic salt solution in the continued absence or presence of 17β-estradiol. Shown are means (± SD) of three to five repeats per point of changes in pHo in the contraluminal (CL-pHo, A) or luminal compartments (L-pHo, B) 30 minutes after shifting cells to basic salt solution. In B, a and b: P < 0.01 compared with SFM.