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. Author manuscript; available in PMC: 2008 May 6.
Published in final edited form as: Methods. 2005 Dec;37(4):341–344. doi: 10.1016/j.ymeth.2005.07.016

Fig. 2.

Fig. 2

Analysis of RNA annealing efficiency and the integrity of dsRNA. (A) A PCR product Xanked by T7 and SP6 RNA polymerase promoters is transcribed with each RNA polymerase to generate single-stranded RNA. These RNA strands are then annealed together to generate double-stranded RNA. (B) Analysis of RNA by agarose gel electrophoresis. The SP6 and T7 transcripts separately synthesized from a vector were run on a 1.5% agarose gel electrophoresis (lanes 1 and 2). These two RNAs were annealed by incubating them together at 78 °C for 10 min and slowly cooling the reaction at 37 °C for 30 min. The annealed dsRNA has a clearly distinct mobility in an agarose gel (lane 3) than either single-stranded RNA. A DNA marker was loaded in lane 4.