FIGURE 2.
Murine AMφ respond strongly to NTHi OMP only in the presence of IFN-β. Resident AMφ from normal C57BL/6 mice were incubated for 6 h in the presence of medium alone, 1000 U/ml IFN-β, 100 ng/ml of each of four OMPs (PCP, P4, P6, and positive control LP) (□), or 100 ng/ml of each OMPs plus 1000 U/ml IFN-β( ■). Supernatants were collected and assayed by ELISA for (A) IL-6 and (B) TNF-α. φ, None detected. Data are mean ± SD of triplicate wells.*, p <0.05, unpaired Student t test. One of three independent experiments with similar results is shown.