Overnight stimulation of murine AMφ with NTHi OMP plus IFN-β increases IL-6 production and induces the chemokines RANTES and IP-10. Resident AMφ from normal C57BL/6 mice were incubated for 18 h in the presence of medium alone, 1000 U/ml IFN-β, 100 ng/ml of each of four OMPs (PCP, P4, P6, and positive control LP) (□), or 100 ng/ml of each OMP plus 1000 U/ml IFN-β (■). Supernatants were collected and assayed by ELISA for (A) IL-6, (B) CCL5, and (C) CXCL10.φ, None detected. Data are mean ± SD of triplicate wells.*, p <0.05, unpaired Student t test. One of two independent experiments with similar results is shown.