Figure 6.
LOH of Apc increases as adenoma size increases. A, adenomas of various sizes and neighboring normal tissue were microdissected out from intestines of ApcMin/+ and Klf4+/−/ApcMin/+ mice. DNA was extracted and the extent of LOH was analyzed by semiquantitative PCR. Adenomas sizes were <1 mm (lanes 2 and 6), 1 to 3 mm (lanes 3 and 7), and >3 mm (lanes 4 and 8). Normal-appearing intestinal tissues (Nl) were also included in the analysis (lanes 1 and 5). B, densitometric analysis of the amplified Apc bands in (A).