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. 2008 Apr 30;105(18):6777–6781. doi: 10.1073/pnas.0802067105

Fig. 1.

Fig. 1.

RNA interference efficiency and the effect of dsInR on ovarian follicle length in C. pipiens. (A) cInR transcript levels in females injected with dsInR were reduced compared with the dsβ-gal controls. Expression levels were measured by RT-PCR 4 and 7 days after dsInR (RNAi) injection, using dsβ-gal as a control and expression of 28S rRNA (30 cycles) as a loading control. (B) Effect of dsInR knockdown on ovarian development of females programmed for nondiapause. Injections of dsInR (≈0.7 μg per female) were made into the thorax of cold-anesthetized mosquitoes within 1 day after eclosion (controls, dsβ-gal), and females were dissected 10 days later. ND, programmed by long day length for nondiapause; D, programmed by short day length for diapause. Both groups were maintained at 18°C. Bars (mean ± SD) with the same letters are not significantly different at P = 0.05, ANOVA.