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. 2008 Apr 30;105(18):6777–6781. doi: 10.1073/pnas.0802067105

Fig. 2.

Fig. 2.

Juvenile hormone rescue of ovarian development in InR knocked-down mosquitoes that were programmed by long day length for nondiapause. (A) Primary (1fol) and secondary (2fol) follicles from wild-type ND females, prepared 10 days after eclosion. (B) Primary follicles from wild-type D females, prepared 10 days after eclosion, showing the cessation of ovarian development. (C) Primary follicles from dsInR + acetone ND treated females dissected 10 days after eclosion. These follicles were arrested in Christopher's stage I, similar to the ovarian arrest observed in diapause. (D) Primary follicles from dsInR-treated ND females that received a 500 ng topical application of the JH analog methoprene in 0.5 μl of acetone, showing nondiapause characteristics, including secondary follicles. (Scale bar, 50 μm.) (E) Mean ± SD length of ovarian follicles in dsInR-injected ND females that subsequently received graded doses of the JH analog methoprene. Each n = 24–32 females. Bars with the same letters are not significantly different at P = 0.05, ANOVA.