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. 2008 Apr 30;105(18):6777–6781. doi: 10.1073/pnas.0802067105

Fig. 3.

Fig. 3.

Knockdown of FOXO with RNAi reduced lipid stores in diapausing adults of C. pipiens. (A) RNAi efficacy in knocking down FOXO expression. Injections of dsFOXO (≈1.0 μg per female) were made into the thorax of cold-anesthetized mosquitoes within a day after eclosion (control, dsβ-gal), and expression was measured 4 and 8 days later (10 mosquitoes per group). (B) Lipid levels (mean ± SD) in females 4 and 8 days after injection with dsβ-gal (control) or dsFOXO. Baseline represents the lipid level 1 day after eclosion. Unpaired t test. (C) Fat females and Nile Red staining of fat body cells in diapausing adult females injected with dsβ-gal. (D) Slim females and Nile Red staining of fat body cells in diapausing adult females injected with dsFOXO.