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. 2008 Apr 24;105(18):6650–6655. doi: 10.1073/pnas.0800739105

Fig. 4.

Fig. 4.

Effect of dsRNA for TcIDGF4 on Tribolium pupal development and pupal–adult molting. (A) dsRNA for TcIDGF4 or vermillion (200 ng of dsRNA per insect; n = 20) was injected into penultimate instar larvae. Normal larval–larval molt and pupation were observed in both dsVer-injected control insects and TcIDGF4 dsRNA-injected insects. Approximately 14 days after injection, the control pupae became normal adults (Left), but the pupae treated with dsRNA for TcIDGF4 were unable to molt and died entrapped in their pupal cuticles (Right). (B) Four insects from each treatment were collected for total RNA preparation. RT-PCR product with a pair of primers for the polyubiquitin gene was used as the internal loading control.