Fig. 4.

Inhibition of the fibrillin-1 network produced by fibroblasts. At the time of seeding of human skin fibroblasts into chamber slides, no protein (A), rF6H multimers (B–D), or rF6H monomers (E) were added at the concentrations indicated. The cells were grown for 3 days, and network formation was monitored by indirect immunofluorescence with antibodies against fibrillin-1. Fibronectin and fibulin-2 network formation are shown as controls in Fig. S5C. (Scale bar: 50 μm.)