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. 2008 Apr 29;105(18):6614–6619. doi: 10.1073/pnas.0710402105

Fig. 2.

Fig. 2.

Effect of β-arrestin-1 knockdown on GLP-1 signaling. (A and B) INS-1 cells were electroporated with scrambled siRNA (Ctl) or β-arrestin-1 siRNA (Arr). Forty-eight hours after electroporation, immunoblotting was performed with anti-β-arrestin-1 antibody or β-tubulin antibody as an internal control (A). The scanned bar graph of β-arrestin-1 blots is shown as percentage of control, normalized by β-tubulin blots from three independent experiments (B). *, P < 0.05 vs. control. (C and D) Forty-eight hours after electroporation with β-arrestin-1 siRNA (Arr) or scrambled control siRNA (Ctl), INS-1 cells were starved with KRBH buffer containing 2.5 mM glucose without serum for 120 min, then stimulated with GLP-1 (100 nM) for 5 min or 10 min, and cell lysates were analyzed by Western blotting with anti-phospho CREB antibody (pCREB), anti-CREB antibody (C), anti-phospho ERK1/2 antibody (pERK1/2), or anti-ERK1/2 antibody (D). The bar graphs are shown as percentage maximum of scrambled siRNA-transduced cells from three independent experiments. *, P < 0.05 vs. control on each time point.