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. 2008 Mar 28;9(5):480–485. doi: 10.1038/embor.2008.37

Figure 2.

Figure 2

Synoviolin interacts with IRE1. (A) Flag-tagged SYVN1 and IRE1 expression plasmids were co-transfected into human embryonic kidney 293 cells. IRE1 protein in the lysates of transfected cells was immunoprecipitated with an IRE1 antibody. The interaction of SYVN1 with IRE1 was detected with a Flag antibody (top panel). The same membrane was reprobed with an IRE1 antibody (middle panel). The expression levels of SYVN1 in the whole-cell lysates were determined using a Flag antibody (bottom panel). (B) SYVN1 protein was immunoprecipitated from the lysate of synovial cells using anti-SYVN1 or normal rabbit IgG. The interaction of IRE1 (top panel) or CHOP (middle panel) with SYVN1 was detected with IRE1 and CHOP antibodies, respectively. The same membrane was reprobed with a SYVN1 antibody (bottom panel). IRE1, inositol-requiring enzyme 1; SYVN1, synoviolin.