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. Author manuscript; available in PMC: 2009 Mar 28.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 22;368(1):145–150. doi: 10.1016/j.bbrc.2008.01.055

Fig. 1.

Fig. 1

Time course of Bax translocation, superoxide generation and CL hydroperoxides accumulation in actinomycin D (ActD) treated MEF cells. MEF cells were treated with 50 ng/mL ActD for indicated period of time (0, 4, 5, 6, 7, 8, 9, 10, 16, and 18 h). (A)(B) Determination of subcellular relocation of Bax in WT MEF cells by Western blot. The levels of Bax were expressed as the mean densitometry ratio of Bax over actin or COX-IV and then normalized to control. (C) Time dependent generation of superoxide in ActD treated MEF cells evaluated by dihydroethidium assay. (D) Time dependent accumulation of CL hydroperoxides in ActD treated MEF cells. CL hydroperoxides were determined using an Amplex Red based fluorescent HPLC assay. Data presented are means ± S.D. (n=3), *(&) p<0.01 (0.05) vs control cells, # p<0.01 vs WT MEF cells under the same condition.