Table 1.

Quantification of alcian blue staining

	Treatment
	None	Gdf5	Noggin	PTHrP
Control cultures	100 ± 1.2	140 ± 6.8**	61 ± 10.3**	113 ± 8.8
Wnt9a-cultures	78 ± 0.3*	109 ± 1.7	21 ± 2.4*	61 ± 2.3*

Images of control (uninfected) and Wnt9a-RCAS infected multiwell cultures stained with alcian blue shown in Fig. 6J and from two additional independent experiments were captured and analyzed by Image J software. Values are expressed relative to control untreated cultures (None) and represent averages and standard deviation.

^*p<0.01 compared with cultures treated with the same factor,

^**p<0.01 compared with control untreated cultures.