Fig. 2.

Cation exchange chromatograms of the dissociation of the two RNase A trimers. The two trimers were heated at 55°C for 5 min, loaded onto a cation exchange Vydac S column, and eluted as described in Materials and Methods. The major trimer (broken line) dissociates into the minor dimer, the major dimer, and the monomer, whereas the minor trimer (solid line) dissociates into the major dimer and the monomer. There is also a trace amount of the minor dimer in the dissociation product of the minor trimer. The pattern of dissociation of the major trimer is consistent with the linear trimer model of Fig. 1A ▶.