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Thermodynamic stability of TCEO (▴) and ECTO (▾) compared with E. coli (□) and T. thermophilus RNases H* (○): (a) Thermal melts in 1 M guanidium chloride (E. coli RNase H thermal denaturation is not reversible in the absence of denaturants); (b) Guanidinium chloride melts, (c) Urea melts. The error bars correspond to errors of fits to a two-state model.
