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. 2002 Feb;11(2):313–321. doi: 10.1110/ps.22102

Fig. 1.

Fig. 1.

SDS-Polyacrylamide gels showing soluble and insoluble fractions after expressing gene number 2 at 37°C using seven different expression vectors. U, T, I, S, and M denote uninduced total fraction, induced total fraction, insoluble fraction, soluble fraction, and size marker, respectively. The insoluble fraction was desolved in a volume equal to that of the soluble fraction. Marker sizes are given in the left column. (A) A 16% gel with Gb1, ZZ, GST, thioredoxin, and 6*His tag fusions, the expected sizes are 17, 26, 36, 22, and 11 kD, respectively. (B) A 10% gel with MBP and NusA fusions, the expected sizes are 52 and 64 kD.