Table 3.
Description of the vectors, enzymes, and reading frame cassettes that were used to create new destination vectors for the Gateway system
| Original vector | New vector | Fusion protein | Promoter | Restriction enzyme | Blunt ends | Reading frame |
| pMAL-c2a | pDEST-TH1 | MBP | tac | NcoI | Nuclease | C |
| pET-43ab | pDEST-TH2 | NusA | T7Iac | SpeI | Nuclease | C |
| pGβ1c | pDEST-TH3 | Gb1 | T7Iac | NcoI | Nuclease | C |
| pT7-ZZAd | pDEST-TH5 | ZZ | T7Iac | BamHI | Klenow | B |
a New England Biolabs.
b Novagen.
c Dr. K. Gardner, Dept. of Biochemistry, UT Southwestern Medical Center.
d Larsson et al. 1996.