Table 1.
An overview of the kinetic study of the ABD variants carried out on the Biacore
| ZABD variant | koff [10−3 s−1] | kon [105 M−1 s−1] | Kaff [107 M−1] | δδG [kcal/mol] |
| wt | 0.6 (0.2) | 1.4 (0.2) | 25.9 | 0.0 (0.2) |
| E3A | 0.6 (0.3) | 1.4 (0.7) | 22.2 | 0.1 (0.1) |
| S18A | 3.5 (1.6) | 1.3 (0.3) | 3.9 | 1.1 (0.1) |
| D19A | 0.8 (0.3) | 1.5 (0.3) | 20.9 | 0.1 (0.3) |
| Y20A | 5.1 (2.1) | 0.9 (0.2) | 1.9 | 1.5 (0.2) |
| Y21A | 3.9 (2.4) | 0.1 (0.07) | 0.3 | 2.6 (0.2) |
| K22A | 1.3 (0.7) | 0.7 (0.2) | 5.6 | 0.9 (0.2) |
| N23A | 0.9 (0.3) | 1.0 (0.1) | 12.7 | 0.4 (0.1) |
| L24A | 0.6 (0.4) | 0.3 (0.02) | 4.5 | 1.0 (0.2) |
| K29A | 1.1 (0.4) | 0.3 (0.1) | 3.4 | 1.2 (0.6) |
| E32A | 2.4 (0.9) | 2.5 (0.3) | 12.2 | 0.4 (0.3) |
| E40A | 1.2 (0.5) | 2.6 (1.5) | 29.5 | −0.1 (0.6) |
| Y20E32A | 17 (11) | 0.5 (0.2) | 0.5 | 2.3 (0.1) |
| Y20E40A | 6.8 (2.1) | 1.4 (0.1) | 2.1 | 1.5 (0.1) |
| S18Y20K22A | 6 | 0.0001 | 0.0002 | 7 |
Standard deviations are given in parentheses. Since the very low affinity of the triple mutant leads to problems in the detection of the binding, no standard deviation is given for that specific mutant.