Table 3.
Thermodynamic parameters for WT* and mutant proteins
| Protein | Tm (K) | ΔH (kJ/mol) | ΔΔGa (kJ/mol) | Std. errorb (kJ/molK) |
| WT* | 313.2 ± 0.7 | 261.0 ± 14.0 | 0.4 | |
| E91G* | 312.3 ± 0.1 | 248.4 ± 1.5 | 0.8 ± 0.1 | 0.2 |
| N92G* | 312.9 ± 1.4 | 249.3 ± 19.0 | 0.2 ± 1.1 | 0.4 |
| H93G* | 321.8 ± 0.1 | 351.6 ± 16.0 | −8.3 ± 0.2 | 0.5 |
| E91G/N92G* | 312.0 ± 0.5 | 247.7 ± 15.0 | 1.2 ± 0.4 | 0.4 |
a ΔΔG = ΔGWT − ΔGMUT determined at the Tm of the wild type. A negative value for ΔΔG indicates a more stable mutant.
b Standard error is for the fit to a two-state model.
* Mutations performed in the amino-terminal his-tagged background protein.