Table 5.
Overview of the heat-induced unfolding experiments
| Reversibility | ||||
| VHHs | Temp. range (°C) | Tm (°C) | CD201–203 nm (%) | KD (native)/ KD (heated) |
| cAb-Lys3 | 25–80 | 62 | 90 | 0.5 |
| cAb-HuL6 | 25–92 | 78 | 91 | 0.63 |
| cAb-NmcA2 | 25–80 | 62 | 20 | ND |
| cAb-BcII10 | 25–80 | 68 | 30 | ND |
| cAb-TEM2 | 25–84 | 76 | 77 | 0.48 |
| cAb-R2 | 25–70 | 60 | 80 | 0.54 |
Tm is the temperature at the midpoint of the denaturation curve, i.e., the melting temperature. Apparent Tm values are given with standard deviations below 10%. KD values were measured using SPR spectroscopy, as described in the text, assuming a homogeneous population of refolded VHHs.
ND: not determined.