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. 2002 Mar;11(3):516–521. doi: 10.1110/ps.39802

Table 1.

Thermodynamic data

Protein ΔTm (°C) ΔH (kcal/mole) ΔΔG (kcal/mole)
WT* 0.00 130 0.00
T157I −5.7 118 −2.05
W158L −4.6 115 −1.75
T157I/W158L −10.3 98 −3.55

Tm is the melting temperature and ΔTm the change in Tm relative to wild type (WT*; see Materials and Methods), the Tm of which is 65.3°C in this buffer. ΔH is the enthalpy of unfolding at Tm and ΔΔG the change in stability relative to wild type, evaluated at an isotherm of 60°C. The uncertainty of ΔΔG was estimated from the standard deviation of ΔG° for WT* to be ±0.15 kcal/mole.