Fig. 4.

The G546D species has protrimer-like mobility. Samples of refolding wild-type phage tailspike (left lanes) were electrophoresed through a native polyacrylamide gel along side cell lysates to permit comparison of the G546D species with the wild-type protrimer intermediate. These samples were electrophoresed in the absence of added reducing agents. The gels resolve both the productive intermediates and the off-pathway intermediates leading to the aggregated inclusion body state. The right-most lane labeled std was loaded with 100 μg/mL-purified tailspike protein. See Materials and Methods for details.