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Global tsf suppressor mutations (su V331A and A334V) do not rescue chains containing the G546D and A575T mutations. Recombinant tailspike chains were expressed in E. coli for 3 h at 30°C. Samples were processed as described in Materials and Methods and the supernatant fractions of cell lysates were analyzed by (A) SDS-PAGE, and (B) native PAGE.
