Table 1.

Data collection statistics for crystals of streptavidin mutants `1' and `2'

Mutant	apo-SAm1	SAm1•StrepII	apo-SAm2	SAm2•StrepII
Protein	streptavidin mutant `1'	streptavidin mutant `1' in complex with Strep-tag II peptide	streptavidin mutant `2'	streptavidin mutant `2' in complex with Strep-tag II peptide
Crystallization conditions	1.2 M (NH4)2SO4, 100 mM Na2HPO4, pH 7.3	1.3 M (NH4)2SO4, 100 mM Na2HPO4, pH 6.9	50 mH Na2HPO4, 50 mM Na-Citrate, 30% PEG 600, pH 7.3	1.2 M (NH4)2SO4, 100 mM Na2HPO4, pH 7.5
Space group	P21	P21	P21	P21
a, b, c	58.42 Å, 86.92 Å, 47.20 Å	58.70 Å, 86.34 Å, 47.45 Å	58.45 Å, 86.81 Å, 47.08 Å	58.39 Å, 86.51 Å, 47.24 Å
β	98.90°	98.92°	98.82°	99.10°
Resolution	1.9 Å	1.7 Å	1.7 Å	1.8 Å
No. of total reflections	52768	126949	178009	120275
No. of unique reflections	33067	49002	48419	41071
Completeness	89.2% (99.00 − 1.90 Å)	94.8% (99.00 − 1.70 Å)	93.8% (99.00 − 1.70 Å)	94.5% (99.00 − 1.80 Å)
	85.5% (1.97 − 1.90 Å)	90.3% (1.76 − 1.70 Å)	89.7% (1.76 − 1.70 Å)	93.2% (1.86 − 1.80 Å)
Rmerge	0.05	0.04	0.07	0.04