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Amino acid sequences of the binding-site loop of wild-type streptavidin and its mutants
| Residue | 44a | 45 | 46 | 47 |
| wtSA | Glu | Ser | Ala | Val |
| SAm1b | Val | Thr | Ala | Arg |
| SAm2b | Ile | Gly | Ala | Arg |
a Numbering according to the mature full length protein (cf. Schmidt and Skerra 1994).
b Voss and Skerra (1997).
