Table 2.
Alanine mutants of BPTI used in the study
| Lys15Arg | R15 | 80%a |
| Pro13Ala, Lys15Arg | A13R15 | 70%a |
| Thr11Ala, Pro13Ala, Lys15Arg | A11A13R15 | 60%a |
| Lys15Arg, Arg17Ala | R15A17 | 70%a |
| Lys15Arg, Val34Ala | R15A34 | 70%a |
| Lys15Arg, Val34Ala, Gly37Ala, Arg39Ala | R15A34A37A39 | 40%a |
| Pro13Ala, Lys15Arg, Arg17Ala, Ile18Ala, Ile19Ala | A13R15A17A18A19 | 40%a |
| Thr11Ala, Pro13Ala, Lys15Arg, Arg17Ala, Ile18Ala, Ile19Ala | A11A13R15A17A18A19 | 30%a |
| Pro13Ala, Lys15Arg, Arg17Ala, Ile18Ala, Ile19Ala, Val34Ala | A13R15A17A18A19A34 | 30%b |
| Thr11Ala, Pro13Ala, Lys15Arg, Arg17Ala, Ile18Ala, Ile19Ala, Val34Ala | A11A13R15A17A18A19A34 | 30%b |
| Thr11Ala, Pro13Ala, Lys15Arg, Arg17Ala, Ile18Ala, Ile19Ala, Val34Ala, Gly37Ala, Arg39Ala | A11A13R15A17A18A19A34A37A39 | 30%b |
Alanine mutations introduced into the primary binding loop are shown in bold and those in the secondary binding loop are in italic. All mutants contain additional Met52Leu substitution. Refolding yield is shown in the third column.
a Refolding conditions: 1 × 10−4 M BTPI, 4 M urea, 20 mM Tris, pH 8.7, 6 × 10−4 M GSH, 3 × 10−4 M
GSSG, 3 mM EDTA, 1 h, 47°C.
b Refolding conditions: 1 × 10−5 M BPTI, 0.4 M urea, 20 mM Tris, pH 8.7, 6 × 10−4 M GSH, 3 × 10−4 M GSSG, 3 mM EDTA, 1 h, 37°C.