Table 2.
Amino acid frequencies in the N-linker, hinge, and C-linker regions of the LacI/GalR transcription repressor familya
| Amino acid frequency | |||||||||||||||||||
| Hydrophobic | Polar | Charged | |||||||||||||||||
| LacI residue number | A | V | I | L | M | P | F | Y | S | T | N | Q | H | D | E | K | R | G | gap |
| 45 | 3 | 3 | 1 | 30 | 1 | 1 | 2 | 1 | 2 | 2 | 3 | 1 | |||||||
| 46 | 2 | 2 | 1 | 11 | 1 | 6 | 4 | 2 | 2 | 4 | 15 | ||||||||
| 47 | 1 | 2 | 3 | 37 | 1 | 1 | 4 | 1 | |||||||||||
| 48 | 2 | 4 | 5 | 1 | 3 | 5 | 3 | 8 | 5 | 4 | 1 | 8 | 1 | ||||||
| 49 | 1 | 36 | 3 | 2 | 2 | 5 | 1 | ||||||||||||
| 50 | 5 | 5 | 7 | 1 | 28 | 1 | 1 | 2 | |||||||||||
| 51 | 15 | 3 | 1 | 3 | 2 | 1 | 3 | 4 | 3 | 2 | 1 | 2 | 10 | ||||||
| 52 | 5 | 12 | 1 | 4 | 2 | 4 | 1 | 1 | 6 | 1 | 4 | 2 | 1 | 1 | 1 | 4 | |||
| 53 | 34 | 1 | 3 | 1 | 2 | 3 | 3 | 1 | 1 | 1 | |||||||||
| 54 | 6 | 3 | 1 | 1 | 6 | 1 | 31 | 1 | |||||||||||
| 55 | 8 | 2 | 3 | 1 | 1 | 12 | 2 | 2 | 4 | 2 | 1 | 2 | 5 | 5 | |||||
| 56 | 5 | 1 | 33 | 3 | 1 | 1 | 1 | 4 | 1 | ||||||||||
| 57 | 13 | 3 | 1 | 4 | 1 | 6 | 2 | 1 | 3 | 9 | 7 | ||||||||
| 58 | 5 | 5 | 1 | 1 | 10 | 5 | 2 | 3 | 1 | 3 | 2 | 11 | 1 | ||||||
| 59 | 2 | 1 | 5 | 1 | 3 | 1 | 6 | 8 | 13 | 4 | 6 | ||||||||
| 60 | 1 | 1 | 1 | 5 | 3 | 3 | 6 | 2 | 3 | 8 | 11 | 3 | 3 | ||||||
| 61 | 5 | 1 | 1 | 9 | 23 | 1 | 3 | 3 | 1 | 3 | |||||||||
| 62 | 1 | 1 | 1 | 1 | 1 | 7 | 5 | 2 | 2 | 5 | 2 | 12 | 4 | 3 | 3 | ||||
| 63 | 3 | 5 | 5 | 2 | 1 | 1 | 4 | 17 | 2 | 3 | 4 | 3 | |||||||
a A BLAST search against SwissProt (Altschul et al. 1990; Bairoch and Apweiler 2000) identified 81 proteins with reasonable homology to LacI. Of these, 50 proteins contain both a DNA binding domain and full-length core and are included in this table.
b Bold numbers correspond to the amino acid residues of LacI.
c Underlined numbers correspond to the amino acid residues of PurR.